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AP Biology Unit 3 Visual Review
A topic-by-topic visual walkthrough of Cellular Energetics — enzymes and their environment, ATP and energy coupling, photosynthesis, and cellular respiration.
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TOPIC 3.1
Enzymes — Biological Catalysts
without enzyme
with enzyme
free energy
reaction progress →
Enzymes LOWER activation energy (Eₐ)
What an enzyme does
Speeds up a reaction by lowering its activation energy —
the energy barrier reactants must cross. It does NOT
change whether a reaction is favorable, only how fast.
Active site & specificity
The substrate binds the enzyme's active site, forming an
enzyme–substrate complex. "Induced fit" — the site molds
around the substrate. Each enzyme is specific to its shape.
Key facts
Enzymes are (usually) proteins and are NOT consumed — one enzyme catalyzes reaction after reaction.
Their names often end in "-ase" (e.g., lactase, DNA polymerase). Cofactors (metals) or coenzymes (vitamins)
may be required helpers for the active site to work.
Enzymes lower activation energy so reactions run fast enough for life — without being used up.
The Review Hub · AP Biology Unit 3
TOPIC 3.2
Environmental Impacts on Enzyme Function
optimum
reaction rate
temperature or pH →
too low
denatured
Temperature & pH have an optimum
Each enzyme works best at a specific temperature and pH.
Beyond that, the protein DENATURES — its shape (and
active site) unfolds, so it stops working.
Concentration raises the rate — up to a point
More substrate or more enzyme speeds the reaction until
saturation — when every active site is full, adding more
substrate no longer helps (the rate plateaus).
Competitive inhibitor
Binds the active site directly, blocking
the substrate. Adding more substrate
can outcompete it.
Noncompetitive (allosteric) inhibitor
Binds a different site (allosteric site) and changes the
enzyme's shape, so the active site no longer fits.
More substrate can't reverse it.
Temperature, pH, and inhibitors all work by changing the enzyme's shape or its access to the substrate .
The Review Hub · AP Biology Unit 3
TOPIC 3.3
Cellular Energy — ATP
STRUCTURE OF ATP
adenine
ribose
P
P
P
high-energy bond
Adenine + ribose + 3 phosphate groups
The ATP ⇄ ADP cycle
Energy is stored in the bonds between phosphate groups.
ATP → ADP + Pᵢ releases energy (hydrolysis)
ADP + Pᵢ → ATP stores energy (from respiration)
Cells constantly recycle ATP — breaking off the third
phosphate powers work, then it's rebuilt.
ATP is the cell's "energy currency."
Energy coupling: exergonic powers endergonic
Exergonic reactions RELEASE free energy (−ΔG, spontaneous)
e.g., ATP hydrolysis and the breakdown of glucose in respiration.
Endergonic reactions REQUIRE energy input (+ΔG, non-spontaneous)
e.g., building macromolecules. The cell couples them: energy from ATP hydrolysis drives the uphill reaction.
Breaking ATP's third phosphate bond releases energy that powers the cell's uphill work .
The Review Hub · AP Biology Unit 3
TOPIC 3.4
Photosynthesis
6 CO₂ + 6 H₂O + light energy → C₆H₁₂O₆ + 6 O₂
Light Reactions
in the THYLAKOID membranes
• Chlorophyll absorbs light energy
• Water (H₂O) is split → releases O₂
• Electron transport chain pumps H⁺
• Produces ATP and NADPH
Photosystems II & I capture light; the H⁺
gradient drives ATP synthase.
Output: O₂ (waste), ATP + NADPH →
Calvin Cycle
in the STROMA (light-independent)
• Uses the ATP & NADPH from stage 1
• CO₂ is "fixed" by the enzyme RuBisCO
• Builds G3P → glucose (sugar)
• Does NOT directly need light
It runs on the chemical energy made in the
light reactions, not on light itself.
Output: glucose (stored energy)
Where it happens: the chloroplast
Light energy is converted into chemical energy stored in glucose. Autotrophs use this sugar to build all their organic molecules.
Light reactions make ATP + NADPH (and O₂); the Calvin cycle uses them to build sugar .
The Review Hub · AP Biology Unit 3
TOPIC 3.5
Cellular Respiration
C₆H₁₂O₆ + 6 O₂ → 6 CO₂ + 6 H₂O + ~ATP (the reverse of photosynthesis)
1 · Glycolysis
in the CYTOPLASM
Glucose (6C) split into two
pyruvate (3C).
Net: 2 ATP + 2 NADH
No oxygen required — this
step is anaerobic.
2 · Krebs Cycle
in the MITOCHONDRIAL MATRIX
Pyruvate oxidized; CO₂ is
released as waste.
Makes 2 ATP + NADH + FADH₂
The electron carriers (NADH,
FADH₂) feed stage 3.
3 · Electron Transport
on the INNER MEMBRANE (cristae)
NADH & FADH₂ drop electrons
down the chain; H⁺ gradient
drives ATP synthase.
Makes ~28–34 ATP (most of it)
O₂ is the FINAL electron
acceptor → forms water.
Without oxygen: fermentation
If no O₂ is present, the electron transport chain stops. Cells fall back on glycolysis + fermentation, which
regenerates NAD⁺ so glycolysis can keep making a small amount of ATP. Two types: lactic acid (muscle,
bacteria) and alcoholic (yeast → ethanol + CO₂). Aerobic respiration yields far more ATP than fermentation.
Respiration breaks glucose to make ATP; the electron transport chain produces the most, and needs O₂.
The Review Hub · AP Biology Unit 3
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How to use the visual review
Spend 30 seconds per slide before clicking next. Look at the diagram, then ask yourself: "Could I draw this from memory and explain it?"
Use the fullscreen button () on desktop for the best experience. Use arrow keys to navigate. Tap "Show all slides" to jump around.
This is great for review the night before the exam — fast, visual, and covers everything you need to remember about Unit 3's energetics.